I have cloned a bacterial gene cluster in an expression vector (pMG36c). Each gene of the insert has its own ribosome binding site (RBS) and start codon. Reading (translation) from vector start codon finds an internal stop codon. But since my all genes have own RBS and ATG, shouldn't it be translated separately as happens naturally in the source strain from where I have isolated these genes? Please share your wise suggestions.

Thanks in advance.

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