Absolute Quantification: Serial dilution resulted in same CT values?

Hello, we are currently working on absolute quantification with SYBR Green qPCR. We excised from a plasmid containing the cDNA, a fragment, which will be amplified by the primers we used in our...

10 November 2020 769 4 View

Healthy cell comparison for breast cancer and prostate cancer?

I am trying to find the most acceptable healthy cell line to compare drug cytotoxicity against breast cancer and prostate cancer cell lines in vitro. Any assistance would be appreciated.

18 October 2020 3,730 3 View

How to sterilize 17-beta estradiol?

I am planning on making working solutions of 17-beta estradiol in DMSO. The drug has been exposed outside of the culture hood. My studies will last up to 7 days, so I am wondering if I should...

08 July 2018 7,845 4 View

What is the best way to differentiate between efferent and afferent sympathetic nerve fibres immunohistologically?

Efferent as afferent renal sympathetic nerve fibers have a important relevance in the development of cardiovascular disease. Thyrosine hydroxylase is a marker for sympathetic fibers. Is there a...

04 June 2018 4,657 2 View

How can I best analyze the change of an insect community composition and diversity over time?

The communities abundances were measured under 3 different treatments, over a time period of 55 days.

06 December 2015 8,027 8 View

How do I treat the transition between excitonic absorption and the Urbach tail?

Dear colleagues! I am wondering if there is any method to properly treat the transition from absorption by gap states (Urbach tail) to excitonic or band-to-band. When modelling all contributions...

29 September 2015 2,731 0 View

What causes Vitamin B1 (Thiamine) deficiencies in native predator fish that rely on a large percent of exotic prey fish in their diet?

08 September 2015 6,268 3 View

Has anyone experienced problems with drying THF and diethyl ether using a solvent purification system (MBraun)?

We are experiencing problems drying THF and diethyl ether with an MBraun SPS solvent purification system. The solvents have the expected low water content (10-20 ppp according to...

03 July 2015 8,464 11 View

How do I simulate Capacitance-Voltage (CV) characteristics of a Schottky contact using COMSOL?

Dear Colleagues, I am currently trying to validate some CV data. Therefore I would like to model the CV characteristics (capacitance at different bias levels) I am new to COMSOL but it is the only...

25 May 2015 7,377 2 View

Has anyone analyzed porcine Cytochrome C via Western Blot?

We are looking for a primary antibody against cytochrome c known to work in pigs. Thank you!

13 April 2015 3,499 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View