I am a graduate student majoring in horticulture.
My first semester just ended and I'm planning to design a simple experiment.
I want to make synthetic DNA that would encodes a short peptide that consists of just 9-10 amino acids.
If I made the artificial DNA with an appropriate promoter and inserted the fragments into the plant (by agrobacterium), would it produce the peptide all over the cell?
Or, if I want to locate the peptides in cytoplasm below cell membranes, what am I to do to make it so?
The DNA fragments would be very short.
I'm curious if it could be simply made in the lab and how much it would cost if I ordered it from a company.
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
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Look up Dov Borovsky. Diet Pill for mosquitoes. We made TMOF, a small peptide that turns off trypsin synthesis in mosquitoes. And other insect spp. Trypsin Modulating Oostatic Factor. It works in flies and other deleterious insects.