I want to extract curcumin and, if it's not pure, I won't be able to use the spectrophotometer in order to prove the presence of curcumin in the extract, because the existence of other substances would affect the measurement.
You need a characterization method to know if you have your compound or not, even if ethanol was able to extract curcumin you need to prove it.
No, other compounds will be extracted. You can use silica gel column chromatography with a dichloromethane: ethanol gradient to purify the curcumin sufficiently for spectroscopic analysis.
See:
http://www.teledyneisco.com/en-uk/liquidChromatography/Chromatography%20Documents/Posters/Purification%20of%20Natural%20Products%20Using%20Mass%20Directed%20Fractionation%20Poster.pdf#search=curcumin
Dear Victor,
Which curcumin you need to extract...?
Curcumin extract containing many other compositions such as tetrahydro curcumin, bisdemethoxy curcumin, demethoxy curcumin etc. You should be very carefull while extracting the pure curcumin because ethanol dissolves all other compositions in it. The best option is, you can purify curcumin just by passing through silica column by using ethyl acetate and and petroleum ether as a mobile phase. finally you should confirm by NMR spectroscopy.
To answer the questions, yes, i want to use spectroscopy and a curcumin standard purity 95%+. I need all curcumins, but nothing else.
yes it is possible by column chromotography. on the basis of RF value its possible to idintify the required product spot. just for information regarding appearence, tetrahydrocurcumin is white powder, bisdemethoxycurcumin is quite similar to brownish red and curcumin is yellow colour. just try to know the RF of each.
See organic process research dev,20(9):1593-1602 (2016) for methods of purifying demethixy curcumin from crude extracts
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