I have placed immature grains of wheat in referigerator sealed in petriplate, after giving 100%alcohol treatment for 2 min to remove contamination and storage for long time (2 weeks). But the embryos from these grains didnt respond to callusing. Is there any method by which immature embryos undergo callusing? May be there is dehydration of embryo in grain by alcohol.
Muhammed Malayan
100% alcohol dehydrates the enzymes in the embryo and the reaction is irreversible.
0 votes
0 thanks
Neha Gupta
Thanks, even I think so as even on nutrient media embryos did not respond.
0 votes
0 thanks
Suman Parre
Cell respond to that and osmolytes produced ,obviously get removed, andtransported to vacuoles
0 votes
0 thanks
Suman Parre
Cell has to respire and then it need some water and macronutrients provide then and temperature
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology
More Neha Gupta's questions See All
Similar questions and discussions