My study is on using microbial DNA present on fingerprints as identity markers of humans. In this case, how high is the accuracy of identifying the phylogenetic DNA of bacteria present on a fingerprint with the use of crude DNA extraction? I am only familiar with the protocol based on other papers - how do I acquire enough DNA for PCR
Abhijeet Singh
1) Its not 16S RNA analysis, it is 16S RNA gene or 16S DNA analysis
2) What do you mean by crude DNA extraction ?
3) You can identify bacterial genus with enough confidence
4) You have to optimise your sample collection to fulfill the amount of DNA required. Insufficient DNA will or may introduce the bias in the richness/diversity/abundance
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