Hello everyone , I'll be conducting phytochemical analysis for my final year project and Im not sure if I shall use calculate for both absorbance reading and the % of the bioactive . Im also not sure which formulas to use to calculate the %phenolic and % flavonoids as well.

For Total Phenolic Content (TPC)- I'll be using be using Folin Ciocalteu Method and gallic acid will be used as standard

For Total Flavonoid Content (TFC)- first qualitative will be done via Aluminium Chloride test then quantitative using quecertin as my standard

Please suggest me which formulas to use for absorbance reading and which % formula

Thank you xx