I am looking for a good (free if possible) online Notebook.
Any suggestions ?
Thanks
Hello, I was wondering whether results using GFP promoter reporter plasmid are reliable as Luciferase. If GFP reporter vectors are as powerfull as luciferase, which vector is best if I want to...
04 May 2016 5,726 9 View
Hello everyone, I have a question regarding OT-1 stimulation and cytokine production analysis by FACS. I have introduced a transgene in OT-1 mice that is supposed to lower the cytotoxicity of...
01 February 2016 5,870 0 View
Hello, I am trying to stimulate OT-1 cells in vitro and but have had a hard time getting a good IFN-g production as measured by FACS. Here is my protocol: I mix at different effector to stimulator...
11 December 2015 5,773 4 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
for eg. transition shock scale
01 August 2024 5,998 0 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...
19 July 2024 4,120 5 View
I would like to use biofilmQ to analyse confocal laser image, how can do?
16 July 2024 7,628 2 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View