I'd be interested to know how you are going to change the flow rate, precisely. What kind of model are you going to use? Mice? Humans? Do you intend to perform in vitro experiments to confirm your in vivo measurements? The kinetics, are they going to range from seconds to hours?
I#d be interested in you methodical approach too! Especially how you plan to measure NO kinetics. We have a nice in vitro approach to pressurize big and small vessels of all kind. Flow control isn't jet there but possible!