Does anyone know the mechanism of trans-splicing introns? We know that introns can be lost by homologous recombinaison with cDNA, but it looks impossible for trans-spliced introns, do you know how such introns can be lost in an evolutionary way?
In the case of a pre-mRNA transcript, primary transcript or hnRNA, introns are excised and ligation of entrons occur to from the mature mRNA. This event is called splicing.
In Eukaryotes, splicing is catalyzed by Splicesome, 5 snRNA subunits + factors complex.
Splicesome assembly occurs de novo on each hnRNA, and introns contains specific sequences that the splicesome complex recognizes.
The problem is not with that kind of introns, but with transpliced introns. Generally, they are ribozymes of group II in chloroplast or mitochondrial genome.