I'II like to identify pathogens in artisanal wine, knowing that this artisanal wine contain also live "good" microorganisms it's tedious to use conventional screening methods. Anyone aware of DNA base method which can discriminate pathogens. Thanks.
1. The article by Wang et al., 1997 can be referred (http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.1997.00300.x/pdf).
2. You can also reffer to the article published by Naravaneni and Jamil, 2005 (http://jmm.sgmjournals.org/content/54/1/51.full).
3. TaqMan probe-based real-time PCR method can be used (Especially useful in detecting Yersinia enterocolitica bacteria in food (Lambertz et al., 2008).
1. The article by Wang et al., 1997 can be referred (http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.1997.00300.x/pdf).
2. You can also reffer to the article published by Naravaneni and Jamil, 2005 (http://jmm.sgmjournals.org/content/54/1/51.full).
3. TaqMan probe-based real-time PCR method can be used (Especially useful in detecting Yersinia enterocolitica bacteria in food (Lambertz et al., 2008).
Yes, using ITS sequence of each pothogen you can detect DNA of microorganism in your sample.
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