Inhibition of Glucose oxidase at higher concentrations of HRP?

09 September 2017 0 7K Report

Hi,

I'm doing an immunoassay using HRP as my secondary label and wells coated with Glucose oxidase. When I add glucose and TMB at higher concentrations of HRP I fail to get a signal.

Is there any evidence for HRP inhibiting glucose oxidase at higher concentrations? I am confused what is going on here.

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