I would like to analyze and characterize a protein in a PPI network focusing on centrality measures mainly using String database and cytoscape program. My basic problem is that even if I know the interactors based on publications (mainly physical interactions) the String database cannot find the some of the interactors (when typing multiple proteins as query), and the generated PPI network does not show those interactors. Moreover, some interactors are shown in the network, however, not connected to the protein of interest even the interaction was described in publication.

If I use the Biogrid database more real interactors are found and the network is generated, but these are only the first neighbours, and the Biogrid does not allow me the change to extend the network and analyze the network as a global measure.

Is there anyone who is familiar with String database?

Thank you in advance!

Kind regards,

Elek