Hello,

in Ghent (Belgium) the team of LabMET is dealing with your topic. http://www.labmet.ugent.be/drupal/?q=node/563

They also founded a spin-off for comercial investigations.

http://www.prodigest.eu/EN/home

Maybe they can help you or at least point you towards another research group.

best wishes

Sir,you should prefere the invitro model for dissolution which directly mimic the digestive system and helps in the study of drug release behaviour of a formulation.

The main problem is that we want to study the extractability/release od some compound that are present in food during the passage along the digestive tract.

You can see this publication. There is a simple protocol to study the extractable of mycotoxin. I think it can be valid for more other substances.

Article Bioaccessibility of Deoxynivalenol and its natural co-occurr...

Dear Dr. Kozubek

I can suggest you some references, but they are focused in microbiology . But they describe deeply the physicochemical conditions in the gut, expecially Macfarlane et al., 1998.

Gibson GR, Rouzaud G, Brostoff J, Rayment N. 2005. An evaluation of probiotic effects in the human gut: microbial aspects. Final Technical report for FSA project ref G01022

http://www.food.gov.uk/multimedia/pdfs/probioticreport.pdf

Macfarlane GT., Macfarlane S & Gibson GR. 1998 Validation of a three stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon. Microbial Ecology in Health and Disease 35, 180- 187.

http://www.springerlink.com/content/rfaa8f3p7tvdvt9r/

I hope you can find something interesting.

Best regards.

More specifically - lipids and lipidic compounds taht are present in food.

Are lipids really digested in +/-50%? Does intestinal microbial flora participate in further availability from food ingested? I have thought about a coctail of enzymes (proteineses, lipases, phospholipases) and bile acids.

Please not to laugh, it isn't my specialization/expertise. I just have the idea to start something new (at least for me).

şaka Evet

Dear Meric Altinoz.

If I knew that all you have mentioned earlier I would not have the reason to ask.

Yes U can model the exact system, in vitro model of catabolism of food stuffs can acquire by controlled in vitro biotransformation.

Dear Dr. Kozubek

I can suggest you these references focused in mycotoxins which I am the first author:

http://www.sciencedirect.com/science/article/pii/S0278691511004832

http://www.sciencedirect.com/science/article/pii/S0278691512004061

I hope you can find something useful.

Best regards.

You could use the standard method for in vitro gastrointestinal simulation test using different buffer systems with its corresponding enzymes. We use this method on our publication based on the method of Chavarri et al. (2010), Marques et al. (2011), and Grimoud et al. (2010).