Hi Jebel Haji Mahamud the answer to your question can be very different based on the circumstance you are in. For instance, the existence of dual emitting modes of the fluorophore can be one reason. Having a optically active (light absorbing and emitting) contaminant or impurity can be another reason. Also excimer formation or aggregate generation can be another reason. Or it can be a nature of the emission band in the particular fluorophore due to fine vibronic structures.