Are negative valued indicators allowed in SEM?

Hello, Using secondary and continuous data, i am trying to run a SEM with two latents. One indicator has both negative and positive values. Is it wrong? (I am gonna run the model in AMOS) Thx on...

14 February 2021 2,801 8 View

How to de-clump HEK293 suspension cells?

I have a problem with clumping of HEK293 suspension cells, and this clumping problem decreases the cell viability. I will transfect the cells if I were to reach a favorable cell count. What can I...

17 January 2021 354 4 View

How to estimate dichotomous variables in SEM?

14 October 2020 5,912 3 View

Is single indicator usage is appropriate in SEM when the data ise continous?

Hello, to use single indicator in SEM , reliability is constrained to cronbach alfa. But when we use continous data we do not calculate cronbach alfa, then can we use single indicator latents for...

02 May 2020 3,455 3 View

In structural equation, Can coefficient between covariate and indicator when controlling latent be estimated ?

Hello, In MIMIC models, can coefficient between covariate and indicator when controlling latent be estimated by direct paths from covariate to indicator? do you mind to take a look at my model...

29 February 2020 374 3 View

In SEM how we favor a model?

Hello, In structural equation modeling can we favor a model over the rival one because of better multiple correlation? I have two rival models. First is slightly have better fit values than...

16 November 2019 5,337 1 View

How many cause variables can we include in a MIMIC model?

hello, I want to run a MIMIC model in AMOS, is there a ideal number of cause indicators? Thanks on advance,

22 September 2019 4,046 9 View

Is scale mean a statistic that can be tested for significance?

Hello, My friend created a hypothesis "Is x (variable) is significant?". I found this hypothesis weird:) Anyway, he insists on testing this. I calculated the mean of the scale (x) on SPSS. Is...

31 July 2019 5,842 5 View

In SEM, can we link egzogenous observed variable to indicator of endogenous latent?

In my structural equation model, I have a an observed egzogenous variable, according to modification indices, can i link it to an indicator of endogenous latent? Thanks in advance

01 November 2018 3,191 6 View

I got a non positive definite warning on SPSS?

I have 40 observations and 32 items and I got non positive definite warning message on SPSS when I try to run factor analysis. Should I increase sample size or decrease items? Or both of them?Thanks

18 June 2018 5,762 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View