In Acetic acid-induced writhing test why we gave extract via intraperitoneal (i.p.) injection when traditionally the extract was consumed orally?
@Samina Nasrin
In actual fact, it is not a rule nor is it advisable to give an extract, that is being tested based on traditional use, via i.p. route. Unless, it is a substance being developed to be administered via the i.p. route. Otherwise, the rule is to prepare and apply the extract in a way very similar to how it is used in the traditional medicine
@Samina Nasrin
In actual fact, it is not a rule nor is it advisable to give an extract, that is being tested based on traditional use, via i.p. route. Unless, it is a substance being developed to be administered via the i.p. route. Otherwise, the rule is to prepare and apply the extract in a way very similar to how it is used in the traditional medicine
exactly this is the case , but also I have Q. here , sometimes 0.6% acetic acid doesn't work ... and I feel that conc. is too low ... what do you think?
intraperitoneal route demands sterile oral dosage form. I doubt if your form is sterile
What is the purpose of this test? I would first like to know the real purpose of such a test, to justify its use at all! If it is to test analgesic drugs, how is this evaluated?
in this test - quote from- Drug Discovery and Evaluation:
Pharmacological Assays - Vogel [Book] " Pain is induced by injection of irritants into the peritoneal
cavity of mice. The animals react with a characteristic
stretching behavior which is called writhing.
The test is suitable to detect analgesic activity although
some psychoactive agents also show activity. An irritating
agent such as phenylquinone or acetic acid is
injected intraperitoneally to mice and the stretching reaction
is evaluated. The reaction is not specific for the
irritant.
In this test both central and peripheral analgesics are
detected. The test, therefore, has been used by many
investigators and can be recommended as a simple
screening method. However, it has to be mentioned
that other drugs such as clonidine and haloperidol also
show a pronounced activity in this test. Because of the
lack of specificity, caution is required in interpreting
the results, until other tests have been performed. Nevertheless,
a good relationship exists between the potencies
of analgesics in writhing assays and their clinical
potencies.
Let's try it on you guys and watch you writhe! Isn't there something better than that? How about doing it in the subjects we really want to know about? Let's start with you guys! Let's see you writhe and evaluate that! There MUST be something better!
Roger Jelliffe MD
It is absolutely wrong to give extract IP to animals. PH , Chemical compositions, particle size , chemical structure ,mole.wt., its volatility, vaporous pressure etc matters route toxicity in animals . So when a particular drug or entity is meant for desired route ,we can not change its route for safety or efficacy studies.
Plant extract may be given orally, because traditionally plants are used orally. for IP route the test substance must comply with the requirements of parentral preparations
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