Shy et al. 2019 mentioned that ABO typing is usually performed at room temperature.
(Reference: Shy, B. R., Tholpady, A., & Nedelcu, E. G. (2019). Interferences in blood bank testing. In Accurate Results in the Clinical Laboratory (pp. 425-437). Elsevier. DOI: 10.1016/B978-0-12-813776-5.00028-5)
Dharmesh Chandra Sharma
Antigen and antibodies reaction is temperature sensitive. ABO group antibodies are best react at room temperature ( Cold antibodies ). Rh D antibodies are worm antibodies best react at 37 degree centigrade. Below room temperature lot of other clinically insignificance cold antibodies will interfere in the reaction .
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