How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

How to make open-top chambers?

Hi, I wonder if someone would share experience with building open-top chambers for surface heating of soil. My main question is what material can be used to build them? I found researchers using...

05 January 2021 5,396 6 View

Is all normalization for microarray the same?

What i know, microarray data has vsn, loess and quantile normalization . i am dealing with GEO microarray datasets where i have to find the differential expresssed genes for NPC. im planning on...

10 December 2020 1,496 3 View

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests?

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests? I am looking for carbon storage potential ie carbon biomass for a number of native trees and shrubs...

08 July 2020 5,236 2 View

Is Linear mixed model appropriate for assessing if there is a difference in tree density between land use types. I have a nested data?

Below is a hypothetical example of how my data looks like in excel. My actual data has 16 sites and each site has about 10 plots, so its a huge dataset). Plots are nested within Sites. I would...

12 April 2020 9,774 6 View

What is the best solvent to dissolve nanoclay (montmorillonite)?

I need to inject nano-clay to some kind of collapsible soil (silty loess) to stabilize and strengthen it. I can't use water since this soil is moisture sensitive and water may dissolve the bonding...

26 March 2020 1,675 3 View

What is the best way to simulate grazing?

Hello, I'm planning to conduct a multi-species experiment to measure different plants' response to grazing. However, I've read that clipping is not a reliable way to simulate the grazing act, so I...

24 February 2020 3,135 9 View

What statistical method should I use to compare environmental variables?

My project is looking at the physical characteristics of 20 different waterbodies which influence bird abundance and species richness. I am looking at surrounding habitat structure and estimating...

01 February 2020 9,342 2 View

Can anyone tell me the largest number of proteins directly extracted from soil ?

Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...

30 January 2020 9,060 2 View

How to determine the primary productivity of a grassland ecosystem?

I am looking for a reliable method to determine the productivity in grassland and forest communities.

15 January 2020 6,592 3 View