I'm isolating hydrocarbon degraders using Bushnell-Haas supplemented with 1% diesel as carbon source.
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I was incubating my tissue with primary antibodies in a solution of PBS, DMSO, Tween20 and 3% milk at 37°C. Unfortunately, the milk started to curdle. Will this affect my experiment drastically?...
14 April 2024 842 2 View
Hello all! I have synthesized hydroxyapatite nanoparticles and have to characterize them with DLS and SEM analyses. Can someone please let me know the sample preparation methodology for DLS and...
06 April 2024 7,614 5 View
I need to require recent publication papers for speech recognition
01 April 2024 4,103 3 View
I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...
11 March 2024 1,000 4 View
Which is the more accurate method to calculate LUMO value for a D-A system? Either it from absorption edge or from CV measurement on reduction side?
15 February 2024 5,635 1 View
We have 4-8 treatment groups and would want to compare the survival curves of the experiments. The log rank test in Prism seemingly doesn't work for more than two groups. Would it be right to...
19 January 2024 1,397 4 View
I am carrying out cell-free DNA extraction using an in-house buffer system and magnetic nanoparticles. My qubit readings and tape station data look good. But upon using some plasma samples (2 out...
11 January 2024 9,426 0 View
This is for my postgraduate research work.
22 December 2023 5,138 2 View
we are applying 24v for 40mins.After that our nc mebrane is becoming like the image i posted, we tried to change current and timing, every time it is becoming like this.
29 October 2023 9,589 0 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...
06 August 2024 8,716 0 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View