"GRADE in a systematic review without meta-analysis?

In a systematic review without meta-analysis, should I necessarily indicate the quality of the evidence (GRADE) for each study included in the review?

31 May 2020 3,400 9 View

Can anyone advice a good (sensitive and specific) commercial antibody to be used in western blots that detects LAMP2 in mouse extracts?

I would like to use LAMP2 to evaluate glycosylation in mouse tissues.

03 April 2019 246 0 View

How to determine the glass transition temperature of pharmaceutical polymers?

Which methodologies using thermal analysis are available to visualize this phenomenon in polymers used in pharmaceutical field?

13 September 2018 2,546 1 View

Which method of data analysis should I use for a study using questionnaires?

I'm conduzing a study about journalists/designers/programmers perceptions about readers when they create a visualization/infographic in online websites. My hypothesis is that the innovative...

24 July 2018 1,363 53 View

How to permeabilize exosomes to evaluate its cargo through ELISA?

Hi everyone, I'm wondering if someone could help me. I want to evaluate the presence of specific proteins in the interior of exosomes but can't find any protocol that would allow us to...

21 July 2016 4,406 1 View

Call differential binding events, peak score (MACS)?

I am comparing 2 conditinos (negative control and treatment) in a Chip-seq experiment. I used MACS 2 (Call differential binding...

05 April 2016 3,914 1 View

Some advice for negative control genes in ChIP qPCR?

14 January 2016 475 2 View

Has anyone got more than 1 band when using abcam Rabbit anti-human CXCR4 polyclonal antibodies?

06 December 2015 4,902 4 View

Can someone help me with MagNA Pure 96 extraction protocols?

We are trying to extract HPV DNA from cervical swabs (ThinPrep) using MagNA Pure 96 equipment from Roche and we are encountering a serie of dificulties. We have tried several approaches regarding...

02 August 2015 2,411 3 View

Should I coat the wells and starve the cells for a scratch assay with Glioblastoma cell lines?

12 June 2015 746 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Should I include less important items in con-joint survey experiment?

I plan to do a conjoint survey experiment in which I randomly change attributes of hypothetical job candidates and show two types of potential candidates with different attributes. Then, I ask...

16 February 2021 5,812 1 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

What would be the best survey design and stat. analysis for my research- impact of pandemic on pro-conservation and pro-environmental behaviours?

Ive started to design the survey with demographics, I have a list of pro-environmental and conservation behaviours which i plan to ask if they have changed since the pandemic. I think i'll use...

15 February 2021 4,204 3 View

Bootstrapping: to use or not to?

Hello, I would need help w.r.t deciding on an approach. I sent out a survey and about 2000 of 15000 recipients completed the survey. Doubts have been raised on accepting the results based on...

15 February 2021 5,585 13 View