what volume of silver nitrate and cell free filtrate of fungi should be mixed to get smaller size nanoparticles?
Dear Asma,
Using the following procedure provides you with small size nanoparticles (5-50 nm),
In addition, please find attached paper that gives you a clear picture on the sizes obtained by different ratio of silver nitrate/cell free filtrate of fungi (Table 1 in the attached paper).
Biosynthesis of silver nanoparticles
In a typical biosynthesis production scheme of silver nanoparticles, 10g of Trichoderma reesei fungus wet biomass was mixed with a 100 ml aqueous solution of 1 mM silver nitrate (AgNO3 ). Then the mixture was placed in a 100 rpm rotating shaker at 28 °C for 120 hours duration. In this process silver nanoparticles were produced through reduction of the silver ions to metallic silver.
Hoping this will be helpful,
Rafik
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