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Introducing a 5% to 10% dilution of a peptide in sterile water can affect Pseudomonas aeruginosa growth, contingent on the peptide's antimicrobial properties. Research indicates that antimicrobial peptides (AMPs) like Cecropin A2 and ABP-CM4N can significantly inhibit P. aeruginosa by permeabilizing membranes and disrupting cellular processes [2][3]. Such peptides, even at lower concentrations, could hinder bacterial proliferation. Conversely, if the peptide lacks antimicrobial efficacy, the dilution may not notably impact growth. Empirical studies are essential to determine the precise effects on bacterial growth rates and colony-forming units under these dilution conditions [1][4][5].

Reference

[1] Mwangi, J., Yin, Y., Wang, G., Yang, M., Li, Y., Zhang, Z., & Lai, R. (2019). The antimicrobial peptide ZY4 combats multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii infection. Proceedings of the National Academy of Sciences of the United States of America, 116, 26516 - 26522.

[2] Hirt, H., & Gorr, S. (2013). Antimicrobial Peptide GL13K Is Effective in Reducing Biofilms of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 57, 4903 - 4910.

[3] Li, J., Zhang, J., Li, G., Xu, Y., Lu, K., Wang, Z., & Liu, J. (2020). Antimicrobial activity and mechanism of peptide CM4 against Pseudomonas aeruginosa.. Food & function.

[4] Ju, X., Chen, J., Zhou, M., Zhu, M., Li, Z., Gao, S., Ou, J., Xu, D., Wu, M., Jiang, S., Hu, Y., Tian, Y., & Niu, Z. (2020). Combating Pseudomonas aeruginosa Biofilms by a Chitosan-PEG-Peptide Conjugate via Changes in Assembled Structure.. ACS applied materials & interfaces.

[5] Pedersen, B. H., Gurdo, N., Johansen, H., Molin, S., Nikel, P., & Rosa, R. L. (2021). High‐throughput dilution‐based growth method enables time‐resolved exo‐metabolomics of Pseudomonas putida and Pseudomonas aeruginosa. Microbial Biotechnology, 14, 2214 - 2226.