Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

What are the existing NLP techniques that can be used to extract use cases from a software requirement text?

according to findings so far Micro understanding is the extracting of individual entities, facts, or relationships from the text. so findings approaches and algorithms to performing that provide...

20 January 2021 8,378 3 View

Could someone please provide me with a reliable differentiation protocol for IMR-32 NB cells?

Treatment with 10uM Retinoic acid seems to result in vacuolization and cell death

20 January 2021 4,013 2 View

How to modeling winkler- Pasternak foundation in Abaqus?

I'm trying to modelize functionally graded material beam resting on winkler- pasternak foundation using ABAQUS, however, I could not find any helpful material.

18 January 2021 4,830 4 View

Is there a typical range in the power spectrum that each person has during a rest measurement? Moreover, are there differences between men and women?

Is there a typical range in the power spectrum that each person has in the individual brain areas (19 electrodes, 10-20 system) and frequency bands (theta, alpha, beta gamma) during a rest...

13 January 2021 5,660 3 View

Why are there radical local increments of contact pressure while the pressure distribution on the rest of the contact region seems logical (ANSYS) ?

I am performing a static structural analysis on a cynindrical roller bearing. The contact stress and Von - Mises stress on the roller surface needs to be aquired. The mesh on the contact region is...

13 January 2021 5,519 2 View

While making prediction with Fuzzy Logic in Matlab, do we have to define the rules for Testing sets also?

I have a dataset with 19 training points and 6 testing points. At first I defined the rules for 19 training sets and then tried making predictions for the rest of 6 points. But I got all constant...

06 January 2021 3,772 5 View

How to I carry out tobit regression on STATA with Simpson index as my dependent variable?

Hello everyone, I am working on the topic; Impact of Income Diversification on the Livelihood of Small Holder Dairy Farmers. One of my objectives is to evaluate the determinants of income...

04 January 2021 1,388 2 View