I usually use Essential 8™ Flex Medium Kit (A2858501) to culture hiPSC but I want to try PluriSTEM® Human ES/iPS Cell Medium (SCM130). Since hiPSCs are delicated cells, I want some information before changing the medium.
I would be also interested in. Have you tried it already to change the Medium?
Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...
09 February 2021 3,383 4 View
Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...
07 February 2021 8,591 4 View
Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...
28 December 2020 3,503 3 View
Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...
14 July 2020 3,828 7 View
Dear All, I am working with thermo fisher H9 cell line, human induced pluripotent stem cell. I made a mutation on them with crispr cas-9 I obtain single colonies and I propagate my clones. I...
13 June 2020 9,046 3 View
Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...
21 December 2019 2,888 14 View
11 December 2019 3,570 6 View
I'm planning to change the components of cell culture media for reprogramming iPSC in order to make it cost-effective for lab research. some research was made in related areas, but my question is...
10 November 2019 9,457 3 View
As question above is there any certain reason that makes human neutrophil progenitors which can produce neutrophils unavailable? I only know HL60 cells and people differentiate neutrophils from...
10 November 2019 7,763 1 View
I am working on differentiating hiPSC into macrophage through EB method, and i wonder how the EB should look like and what are the characteristics before it is cultured with monocytes...
10 November 2019 3,465 2 View