I usually use Essential 8™ Flex Medium Kit (A2858501) to culture hiPSC but I want to try PluriSTEM® Human ES/iPS Cell Medium (SCM130). Since hiPSCs are delicated cells, I want some information before changing the medium.
I would be also interested in. Have you tried it already to change the Medium?
Hi! I would like to know which restriction enzymes do NOT CUT mitochondrial dna.
09 November 2023 1,918 0 View
The cells are cardiomiocytes and I used Human Cardiomyocyte Immunocytochemistry Kit (Catalog number: A25973; Thermo Scientific). I want to value the different expression of TNNT2 and NKX2.5...
28 November 2021 3,837 2 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
This shape gradually spreads. Even if you install new cells again, it will change like this at some point. Has anyone experienced the same thing as me? I tried using Matrigel again by aliquot it....
12 June 2024 7,269 5 View
Hello everyone. I am culturing hIPSC in mTeSR-1 medium. I found some small round white particles persistently attached to cell colonies, and some actively moved under the 40X objective. Also,...
08 June 2024 6,797 5 View
Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....
19 May 2024 3,164 2 View
Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...
02 May 2024 6,314 1 View
Hello everyone, I work with skin cells and am performing RT-qPCR of primary fibroblast cells and primary keratinocyte cells to further use as a comparison to iPSC-derived skin cells and 3D skin...
22 April 2024 6,181 2 View
Hi all, In my lab we are currently differentiating neurons from hiPSCs according to the protocol used in Satir, T et al, 2020 (doi: 10.1038/s41598-020-57516-7). We have been successful in the...
27 March 2024 7,900 0 View
Hello everyone, in our team we have established the use of NGN2 to differentiate hiPSC into neurons. We do so by transducing neurons with two lentiviruses, one with inducible NGN2 and puro...
07 March 2024 7,758 6 View