I want to use EDTA as a capping agent but EDTA is not water soluble how to use it as a capping agent for synthesis of copper nanoparticles?

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How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavengin?

Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavenging? Please share the files with complete...

30 November 2020 4,935 1 View

Can you help me figure out how many EDTA I need to add in the solution?

Composition 2X Loading dye for RNA electrophoresis 95% formamide 0.025% SDS 0.025% bromophenol blue 0.025% xylene cyanol FF 0.025% ethidium bromide 0.5 mM EDTA. I wanna to prepare 10 ml of the...

10 November 2020 4,759 3 View

Could bone tissue be saved in 70% EtOH after decalcification before processing?

I got the mouse tibia and femur tissue harvested, fixed with formalin 48h and decalcified with 14% EDTA 72h before paraffin embedding. This protocol works well for both HE and IHC. Now, for the...

18 October 2020 5,318 1 View

Does anyone experienced EDTA salts precipitation in DMSO?

I added 2 mL of 0.1 M EDTA aqueous solution pH 6, to a solution of 3 mL pure DMSO and instantaneously I observed salt precipitation in solution. Is this happening because the pH of the EDTA...

17 October 2020 6,329 1 View

How do you create a 0.5M EDTA disodium salt solution at pH=7.4?

I have been trying to make a 0.5M EDTA solution by dissolving EDTA disodium salt dihydrate in 500mL of purified water and adjusting the pH to ~8.0 so that the salt dissolves. To aid in the...

12 October 2020 3,242 2 View

Does anybody know whether it is possible to culture from frozen EDTA whole blood?

Dear all. I am about to start B cell immortalization experiments with EBV, but the vast majority of my blood samples come from frozen whole blood in EDTA tubes and without any cryopreservation...

07 October 2020 7,110 2 View

How do I remove all of the cobalt off of TALON beads?

Hi All, I'm regenerating TALON beads in bulk for the lab. As I've done with Nickel beads for years, I washed with water, eluted all the protein, removed imidazole, and then added 0.2M EDTA pH 8.0...

22 September 2020 4,259 1 View

How can i increase the pH of synthesic sea water to 12?

How can i increase the pH of sea water to 12? Because when i adding NaOH to seawater the pH does not go above 10 and begins to precipitate.

12 September 2020 564 9 View

Which is the best stripping method for proteins having molecular weight 90kDa and above?

Hi, I would like to know which is the best stripping method for proteins having mol. wt. around 90 kDa or more. I am using human samples. I used 0.5M EDTA stripping buffer by boiling PVDF...

08 September 2020 5,815 2 View

0.5M EDTA buffer preparation: Why does it turn into milky white foam-like mixture?

I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...

03 June 2020 4,581 3 View