I have GC -FID without headspace. I want to test Fatty acids from C4 to C24 including cis and trans isomers. Would anyone suggest the best extraction method for the FAME test? May I use heptane instead of Hexane?
Please check my published work regarding the analysis of Fatty acid methyl ester (FAME) and its extraction methodology from food products and oils;
Article Formulation of plant based insecticides, their bio-efficacy ...
Some international methods:
https://www.iso.org/standard/74350.html
http://store.uni.com/catalogo/en-iso-12966-2-2017?josso_back_to=http://store.uni.com/josso-security-check.php&josso_cmd=login_optional&josso_partnerapp_host=store.uni.com#
http://store.uni.com/catalogo/en-iso-12966-3-2016/
Thanks for your answers to both of you, but I can not access any of the mentioned documents.
Hi Jainita Teraiya
you can also google "fatty acid methyl ester researchgate" or "fatty acid methyl ester analysis"
Best regards,
roberto
Hi
The detection and calculating protocols are well explanined in the following paper:
https://doi.org/10.1007/s12161-021-02203-0
I hope this helps!
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