Which analytical techniques will be the good option for the identification of unknown compounds from the crude methanolic plant extract?
Dear Ajeet Singh
You can do the analysis using GC-MS if it is non-polar fraction and you are comparing with some know compounds in literature.
Sine it is methanol fraction (polar fraction); one need to do LC-MS and compare with known compounds database.
If you are expecting a new compound then the other method is activity guided fractionation of crude extract followed by identification of purified compound using IR, NMR, MASS, CHN etc.
Dear Ajeet Sing,,
Identification of unknown compounds from the crude methanolic plant extract may be done using GC-MS analysis (qualitative) by comparing data with the NIST & Wiley library..
Dear Ajeet Singh
You can do the analysis using GC-MS if it is non-polar fraction and you are comparing with some know compounds in literature.
Sine it is methanol fraction (polar fraction); one need to do LC-MS and compare with known compounds database.
If you are expecting a new compound then the other method is activity guided fractionation of crude extract followed by identification of purified compound using IR, NMR, MASS, CHN etc.
Dear Madam,
How I identify/confirm unknown compounds from extract fraction using LC-MSMS as there NIST & Wiley library is absent ?
I think this was asked in two places? Please see my answer in the other instance of this question...literature search on you plant.
It depends on the the fraction, nonpolar, or polar, volatile or nonvolatile, you can use the GC-MS with the NIST or LCMS with the library to compare,
It depends on your plant extract because the every test materials by LC-MS or HPLC, GC-MS give different, molecular weight and structure of the components .
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