The antioxidant power can be evaluated in vitro or in vivo by applying methods that measure the antioxidant capacity of a molecule or a natural extract. Given the complexity of the oxidation processes and the diverse nature of antioxidants, with both hydrophilic and hydrophobic components, there is not a universal method by which antioxidant activity can be measured quantitatively in a way very precise. Most often, the answers of different and complementary tests must be combined to obtain an indication of the antioxidant capacity of the sample to be tested.

Please see these articles:

https://www.researchgate.net/publication/232784476_Antioxidant_Determinations_by_the_Use_of_a_Stable_Free_Radical?_iepl%5BgeneralViewId%5D=80FBSjbLKv70dgyK0ifXYqVUabEHoeyC1o3H&_iepl%5Bcontexts%5D%5B0%5D=searchReact&_iepl%5BviewId%5D=a9uJPUbQ3HYTbscMH7LkHR8fvXNuARr35VT1&_iepl%5BsearchType%5D=publication&_iepl%5Bdata%5D%5BcountLessEqual20%5D=1&_iepl%5Bdata%5D%5BinteractedWithPosition1%5D=1&_iepl%5Bdata%5D%5BwithoutEnrichment%5D=1&_iepl%5Bposition%5D=1&_iepl%5BrgKey%5D=PB%3A232784476&_iepl%5BtargetEntityId%5D=PB%3A232784476&_iepl%5BinteractionType%5D=publicationTitle

Article Chemical composition, in vitro antiparasitic, antimicrobial ...

Data SUPPLEMENTARY MATERIAL of paper : Chemical composition, in v...

Antioxidant activity can also be determined by the help of H2O2 (hydrogen peroxide) radical scavenging method.

This article will help you.

Hi Asuma Jacob better to use spectophotometer to evaluate the antioxidant activity and their effects to body

Ausama Ayob Jaccob You can get detail protocol based on UV from these publications. Thanks.

Article Preliminary pharmacological evaluation of Alocasia indica Schott tuber

Article Bioactivity studies on Musa seminifera Lour

Article Antinociceptive and Antioxidant Activity of Zanthoxylum budr...

Article Bioactivity studies on Zanthoxylum budrunga wall (Rutaceae) root bark

thank you so much

hello

DPPH is the simple, easy and cheap

if you need any help I will do

interested

Follow

by using DPPH Radical Scavenging assay

Thank you so much

Dear Dr. Ausama, this is a very nice review that comprehensibly covered most of the currently used in vitro and in vivo antioxidant assay methods.

Review on in vivo and in vitro methods evaluation of antioxidant activity

• April 2013, Saudi Pharmaceutical Journal 21(2):143–152

Dear Sir,

I think so, this file will help a lot in evaluating the antioxidant compounds.

You can get details in the attached search

Article Chemical Composition and Antioxidant Capacity of Eggplant Pa...

• DOI:
• 10.1088/1742-6596/1294/9/092013

Agree with Ahmed Burghal