is flconazole really soluble in water...? in which tybe of water? should it warm or normal?can we dissolve it any other solvent like DMSO in place of water,to determine it's MIC.
Poorly soluble in water but can be dissolved in organic solvents such as chloroform, propylene glycol, and polyethoxylated castor oil
Flconazole: Solubility : slightly soluble in water, freely soluble in methanol, soluble in acetone.
Descriptive Term Parts of Solvent Required for 1 Part of Solute
Very soluble Less than 1
Freely soluble From 1 to 10
Soluble From 10 to 30
Sparingly soluble From 30 to 100
Slightly soluble From 100 to 1000
Very slightly soluble From 1000 to 10,000
Practically insoluble, or
Insoluble 10,000 and over
Regards
Selvan
Descriptive Term---------------------------- Parts of Solvent Required for 1 Part of Solute
Very soluble ----------------------------------Less than 1
Freely soluble -------------------------------From 1 to 10
Soluble ----------------------------------------From 10 to 30
Sparingly soluble ---------------------------From 30 to 100
Slightly soluble ------------------------------From 100 to 1000
Very slightly soluble ------------------------From 1000 to 10,000
Practically insoluble, or
Insoluble-------------------------------------- 10,000 and over
MIC is minimum inhibitory concentration. If it's methanol soluble, DMSO is another good carrier, BUT concentrations above 1% DMSO are toxic/inhibitory to eukaryotic cells. Thus, you may need to initially dissolve it in DMSO, ethanol, or methanol and serially dilute it to titrate out the DMSO. This may affect the stability and solubility, but if done in gradual gradients, is usually reasonably effective.
Dear Kapila Sengar,
Please ask me if anything analytical Chemistry related for your project. i am sorry i am not aware of the word "MIC" and below link of USP and EP monograph may help you
http://www.uspbpep.com/usp32/pub/data/v32270/usp32nf27s0_m33240.html
http://www.uspbpep.com/display.asp?sel=ep60&filename=fluconazole%202287e
Regards
Selvan
@Rajeev: my understanding from what he wrote is that he would like to establish the MIC dose of fluconazole, but first needs to dissolve it in water (or saline solution or cell culture media) to be useful. For substances that are not water-soluble, that may require some effort. I don't know what target organism this is intended for, either.
@kapila. If you are interested in dissolving Fluconazole for MIC, then you have to use only Water (sterile, distilled water). DMSO is used for other azoles such as itraconazole, vori etc. and also for polyenes - Ampho B. If you are planning to study MIC, you have to follow an acceptable guideline which could be from CLSI (formerlty NCCLS) or EUCAST. again you havent specified what your test fungi are, so you have to selected the appropriate CLSI (mostly followed world over) or EUCAST (more in the europe) document. in that document you will get the scheme for preparing various drud dilutions etc. Alternatively you could also procure ready-to-use MIC plates from commerical manufacturers (look up in the web). you need to only add the broth, inoculum etc and set up the assay.
Gud luck for your work!
thank u all of u for guiding me....C.P. Girish sir..i m doing the same as u r telling me....i m following the CLSI guideline, bt my fluconazole drug is not solouble in water, acc to CLSI....that's why i m using DMSO....is it right to use DMSO as solvent for fluconazole....what should i do now....gv me rply....i m wrkn on fungus candida,,,and determining the mic of fluconazole and ketoconazole on it...
@Kapila. I think there is some issue with your drug in terms of solubility!...What is your source of fluconazole? for MIC you cant be using pharamceutical preparation like tablet/capsule...you have to use pure compound which ussally we get from the manufacturer of the drug or for some like amphotericin B etc. other suppliers like sigma also provide. the important thing you have to check is 'drug potency'. if you are indeed using an CLSI document, i think details of drug potency should be given . anyways remember pharamaceutical preparations have lower potency than pure compound. for eg., A 500mg fluconazole not only contains fluconazole but other consistuents like binder etc. all these result in potency around 60-80% whereas pure compound will have 95-99% . based on potency only we determine how to prepare drug dilutions ( formulae should be there in the CLSI document!)and only then can we correlate MIC with susceptibility or resistance.
Also separate guidelines are available for yeasts - M27A3 i suppose is the latest one. you have to have the complete/standard document which will have the whoile procedure. there are in addition supplementary documents which have only updates.
Lastly am just interested to know why you looking at ketoconazole? and not itraconazole or voriconazole!
slightly soluble in water
https://pubchem.ncbi.nlm.nih.gov/compound/Fluconazole#section=Solubility
Poorly soluble in water but can be dissolved in organic solvents such as chloroform, propylene glycol, and polyethoxylated castor oil
referring to the CLSI - M27-A2 document, water is a good solvent for the fluconazole....I suggest using pure Fluconazole that may solve your problem..... good luck
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