Phosphorus element is one of essential nutrients of a plant needed for growth and development. However, in tropical soils, its mineralization mechanism is not very clear. How many forms of P available to the plants and how many forms are still remained e.g. organically bounded, geologically bounded etc. that do not take role in the mineralization, therefore, demand and supply in tropical soil especially in agro-ecosystems are very mysterious and condition always needed sublimate in form of chemical fertilizer that makes not good sense for ecologically aesthetic So, I wish to get most authentic and appropriate protocols for analyses.
Dear Anand, The Tian et al paper is one of the latest that gives information on fractionation of P forms. All the best.
Dr. Singh, the following methods you may try, I hope you may get good results under tropical soils.
1. Inorganic P fractions : Kuo, (1996) and Tarafdar et al. (2006)
2. Organic P fractions : Bowman and Cole (1978) and Tarafdar et al. (2006)
3. Available P : Olsens et al (1954) - As soil your place mainly neutral to alkaline
4. Total P in soil : Olsen and Sommers (1982)
5. Phytin P : Mega (1982)
Dear Sir Thanks,
Yes I have analyzed Total P and available Pi by Olsen et al (1982) and Molybdate method (1954). Results are not very satisfactory and accurate, however, I will try as recommended. I will focus specifically on P mineralization mechanisms.
With Kind Regards
Dear Anand,
What do you mean when you say that the 'results are not very satisfactory or accurate'? Are you referring to the chemical results or their prediction, e.g. P-uptake by plants or their requirement for fertiliser? If the former the there is a technical problem: the latter is the norm unless the test is 'calibrated' for the soil type.
Paul
Dear Mr. Anand.
Use at least 4 or 5 methods of chemical P fractionation. Soil conditions always vary so fractionation will give you the idea of the P forms and it can be confirmed by spectroscopy technique such as FTIR, NMR, EXAFS etc. Dissolution of the P will also give you the idea of P mineralization mechanism.
There are several papers on these techniques are available.
Dear Dr. Singh,
thanking you for your nice Q. its help us to the estimation of same.
@ Paul Milham, @ Umair Toor @Vijay Singh Meena,
Dear Sir,
I am interested to know rate, availability, dynamics, mechanisms of Phosphorus in Tropical soils. Indeed, values reported for any form of P from tropical soils are unpredictable, there is no trend, no sequence, moreover, tropical soils are P deficient soils!. There are several reasons for deficiency but how plants are getting and accumulating if the soils are deficient. So a question arouse that it might be some other fractions of P which is not determinable or measurable found in the soil play big role in P mineralization.Therefore, I am looking all kind of protocols be analyzable P- fractions, their forms either in mineral or in organically bonded (of course geologically too). So I want to investigate P-,mechanism under contrasting soils of lower Shivalik region (north-western tropical region) of India. In this region, anthropogenic activities like land use, intensive agriculture, urbanization, Biological invasion are going with unprecedented rates.
I will see nature and properties of P in contrasting soils of this region and correlate with Ecosystem functioning behaviour and its services under changing environment.
I wish more encouragement and critical suggestions from our scientific fellows.
Sincerely
Anand
Dr.Singh very good work on the aspects mentioned by you has been done at Punjab Agricultural University ,Soils Department by Dr.G.Dev and his co-workers and students.You may also look into my contribution/ papers in researchgate to find some papers of our team pertaining to your interest.
Dear Sir (Dr A S Rao)
I will get research articles from your Research-gate and also try to get publications from Dr G. Dev, PAU, Ludhiana. I want to analyse P mineralization and mobility in soil to understand cycling of P in Shivalik ecosystem. I know that, there are hundreds of methods available for analyses today (manual and instrumental) but how many fractions and forms of P either in mineral or any other organically bounded could release P elements, how many kind of microbial niche paying trade-offs with plants for P mobilization . Is the mechanism for P mineralization is probably similar for native and invasive plants then how cycling of nutrients (especially N and P) differently behave under invaded ecosystems? In Shivalik region, invasibility by certain invasive species under agriculture and forest ecosystems is a big ecological challenge. I am looking answer through plant functional traits and cycling of nutrients systematically . In this direction, I have to investigate mechanistic role of N and P retention, deposition, cycling and distribution pattern in soil across contrasting ecosystems of Shivalik region in a changing environment. I am very thankful to you for your kind help and suggestions.
Dear Dr.Singh, your comments indicating the nature of your work are interesting.I shall be able to provide some pertinent references in the first week of June.I wish you success in your work.
Dear Sir,
I am delighted to get your kind and prompt reply. I will be very obliged to you for your kind support.
With kind Regarfs
Sincerely
Anand
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