What are the commonly used housekeeping/constitutive genes in bacteia for gene expression studies?

I want to study the expression level of some genes in Bacteria, what is the most popularly used housekeeping genes picked as a control. Thank you.

01 February 2018 8,071 8 View

What is the best way to locate the gene from the genome in Bacteria?

I need to find the location of the gene associated with particular character from the genome, also I have no clue weather the gene is present in plasmid or in chromosome of bacteria. Can someone...

31 December 2017 1,846 9 View

How can i make working culture from glycerol stock.?

I have Glycerol stock(6monts old) i need to prepare a working stock. what would be apropriate method for this. should i streak over plates n culture them on broth or i can directly culture them on...

06 July 2014 9,136 3 View

Why can I not measure current and voltage both in parallel by using two multimeters?

06 July 2014 3,193 10 View

Is platinum a better catalyst?

In a MFC Platinum were coated over Carbon electrode, but the electricity produced by Platinum caoated electrode was four times lower than that of plain carbon electrode.

05 June 2014 4,303 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View