my major is entomology and i need full protocol with procedure about the extraction of amino acid and Glucosinolates from plant leaves
i need latest procedure
if anyone knows kindly inform me
Have you checked the net-based resources on the extractions? You could buy them from the commercial suppliers (Sigma, Aldrich, Fluka, Fisher etc.) as extraction will be costly, need the expertise to isolate and purify for entomological purposes.
Hi Ahmed, regarding glucosinolate and secondary metabolites extraction you can check these papers:
Kliebenstein DJ, Lambrix VM, Reichelt M, Gershenzon J,Mitchell-Olds T (2001) Gene duplication in the diversification of secondary metabolism: Tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis. Plant Cell 13:681–693
https://www.researchgate.net/publication/260117112_Brassica_Plant_Responses_to_Mild_Herbivore_Stress_Elicited_by_Two_Specialist_Insects_from_Different_Feeding_Guilds
Article Brassica Plant Responses to Mild Herbivore Stress Elicited b...
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