Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

I've my Gold Nanorods in solution form and I have to conjugate them with drugs. Please go through description i have given?

I've my Gold Nanorods in solution form and I have to conjugate them with drugs. They remain in solution form after conjugation. Now for MTT assay for drug delivery on cancer cell lines, I am...

16 January 2021 3,992 1 View

Hello, can i learn amount of Chlorophyll in Spinach as a mg in 100 grams ?

I have looked on the internet and website but i could not find it so i need yours help please.

26 December 2020 8,354 3 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

I put a sodium chloride which is less than solubility chart but it doesn't melt. can you tell me what the problem is?

24 September 2020 224 3 View

How do I determine the amount of enzyme needed to liquefy a known quantity of starch substrate?

I have an enzyme preparation (powdery form) with an activity of 1045 U/g which I would like to use in mashing a 70 g of grain whose starch percentage is at 67%. How do I determine the quantity...

16 August 2020 1,998 5 View

How to find the densification of any electrolyte for solid oxide fuel cells/ How to calculate it ??

Most of the researchers used Archimedes principle to calculate its density. But that is not 100 % accurate. I want to calculate the density of pellet which is made by using 0.4-0.5 grams powder.

07 August 2020 1,049 4 View

Can anyone share with me their protocol for Picric acid for Pico Sirius Red?

1) How do I dissolve the Sirius Direct 80 Red? I know it should be in saturated Picric acid solution. But how many grams of picric acid should I add to dissolve in water? What should the...

22 March 2020 233 3 View

Why is excel convert function from tbs to gm not working?

hello, I wanted to convert tablespoon measurements into grams in excel (for a whole column), however, it didn't work and gave me an error, so is there any way to convert liquid measurements to...

10 January 2020 2,993 1 View

Does anybody have an Android ".csv" Malware Data-set?

17 December 2019 761 3 View