I need an aliquot of U87 o U87MG cells anybody could help me?

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Does anyone have this article in PDF and could they pass it on to me?

Paper: Wang, A.G., Luo, G.H., 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol. Commun. 6, 55–57.

19 June 2024 7,283 0 View

¿Qué equipo de resonancia de plasmones de superficie recomendarían y cual no para mediciones de interacciones péptido-proteína y péptido-célula?

He visto varias referencias de Biacore y todas mencionan sus ventajas. Sin embargo, nunca he usado uno, por lo que me gustaría recibir recomendaciones de personas que conozcan la técnica y que la...

16 April 2024 4,774 1 View

Are SPL 96 well white plates autoclavable?

Hello, my question is if SPL well white plates (30196) are autoclavable? I didn't find any information in Technical Data Sheet. Thank you for your answers.

25 March 2024 9,903 2 View

What is my ID Research Gate nomber?

Where i cant find my ID Research Gate number?

17 November 2023 1,104 0 View

Manders' Coefficient for Colocalization, tM1 or M1 value?

Hi there, I am trying to assess colocalization (overlap) of two signals. The experiments were performed on FFPE human brain aged tissue samples, which means I have a lot of background after...

04 October 2023 2,877 2 View

How to make an RNase decontamination reagent?

Hello, I hope you are well, I have been searching and I cannot find the components to make an RNase decontamination reagent in the laboratory, without having to buy the RNase away. Thanks

26 September 2023 5,360 1 View

Which models can I use to build growth curves for gmelina arborea?

I am working on the development of growth curves for Gmelina arborea for the northern and Caribbean region of Costa Rica, I am interested in knowing what models you recommend for its construction

20 June 2023 279 0 View

No bands in electrophoresis?

Hello, I'm doing a SOE PCR. As soon as the PCR is done, I run electrophoresis of the samples. The gel shows the marker but no sample, as if I didn't load the sample with the loading buffer. But...

27 April 2023 6,495 9 View

Does anyone have tips on technologies that can capture images and voice of pets?

I'm starting a research on technologies that can capture images and voice of pets, according to your behavior. Can anyone give me tips for articles or tools that help me in this study?

23 April 2023 8,190 2 View

K-40 from K total determined by ICP MS in sediment samples?

Is it possible to mathematically calculate K-40 from K total determined by ICP MS in sediment samples?

31 March 2023 5,428 3 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Has anyone used or is familiar with an anti-C4 complement factor antibody for Western Blotting?

Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...

17 June 2024 6,124 1 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

13 May 2024 3,061 11 View

Why does soybean half-seed transformation use 1/10x B5 salts during co-cultivation?

I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...

07 May 2024 3,924 0 View

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

04 May 2024 6,463 0 View

What are the genetic mechanisms governing the production of secondary metabolites in microorganisms?

How are these exploited in biotechnological applications?

07 April 2024 566 5 View