Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

How to calculate the number of degrees of freedom in a Mantel test?

Hello, I've performed a Mantel test between my microbiological data and the geographic distance where those samples were collected, using a table with 516 samples. However, the test doesn't...

16 January 2021 8,713 4 View

Which are the Non Small Cell Lung Cancer markers?

I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...

04 January 2021 7,986 3 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

Sankey Diagram: Waht is the sample size needed?

Hello everyone. I would like to ask your help on the decision about the best methodology to determine the sample size to conduct an observational study. This is a study to be carried out by a...

16 September 2020 9,014 1 View

How to convert, excel files into (.vgd) or (.vms), ( .dts), (.dpr), (.vgl) files ?. Is there any software is available for this conversion?

I need a compatible file with Avantage for XPS of Themo Scientific but y only have excel files (.xls)

07 September 2020 215 3 View

Any expert on air pollution and its consequences on soil, plants and food production that is available to answer some questions?

Hello, I am a 3rd year Journalism student at Solent University. I am currently working on my Final Major Project. My project is about how air pollution influences our environment, and I will...

16 February 2020 6,332 2 View

I don't get a synthesis sample by pcr, what could I modify? considering that I already started from a standard protocol, I still don't get samples?

am carrying out a final point pcr from a standardized protocol, but I don't get results. I have modified concentrations of magnesium dichloride, DNTPS, DMSO, change the reaction water, the quality...

06 November 2019 7,767 2 View

In the sphere of education for the third millennium could education and inclusive school be an answer?

An inclusive education implies the development of inclusive societies. The inclusive, open-minded school for all educators and everyone, flexible and anchored in solving any educational problem...

07 March 2019 5,999 6 View

How to read the results from Illumina MiSeq machine?

Hi, everyone, I sequenced some samples in the Illumina MiSeq, got the results but now I don't know how to analyze them. I already read the User guide from Illumina, but it's only about the...

02 December 2018 9,374 3 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View