I have run 10% PAGE of Small RNA's qPCR Products. why the gel bands are w and not linear.?

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Ahmed M. Soliman

The "wavy" or inconsistent bands on a 10% PAGE gel for small RNA qPCR products could stem from a few factors:

Voltage and Gel Running Conditions: Running the gel at 50V may not be optimal. Higher or more consistent voltage settings can sometimes improve band sharpness and linearity.

Gel Concentration: Double-checking gel concentration and polymerization can help ensure uniformity.

Buffer Quality: Fresh and properly prepared buffers can prevent uneven migration, which might be causing the wavy bands.

Adjustments in voltage or buffer might resolve the issue, or consider troubleshooting with a control sample to confirm uniform band migration.

Haidar Fayoud

You may have used too much of coloured dye (loading buffer), leading to very different sample ion concentration from that of the buffer in the gel it is running in. Try reducing loading buffer`s volume.

Amy Klocko

Do you flush out the wells before you use them? That can help remove any bits of poorly polymerized gel and improve the shape of the bands.