It is difficult to obtain a representative subsample of crucifer plants with high biomass in order to analyze their glucosinolate content when big lyophilizers are not available, and at the same time to inactivate endogenous myrosinase to avoid glucosinolate degradation.
one of my colleague was working on myrosinase enzyme in same plant family as well as glucosinolate content. I conveyed this question to him directly. He was using liquid nitrogen in large volume, and then keeping at 80C... Why dont you use liquid nitrogen...?
How about sulphate?
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220411/pdf/10417337.pdf
Hi all,
for glucosinolate extraction, lyophilisation of frozen material is method of choice, because freeze-dried material is easy to grind, Myrosinase is inactive without water and the glucosinolates stay intact. Grinding the frozen material under liquid nitrogen might work for leaves, but will be difficult in case of large scale root or rhizome material (wasabi, radish). If material starts melting, degradation of glucosinolates will start immediately. In this case you could try heat drying or boiling before grinding, but consider that glucosinolates are not completely thermo stable. Since you have to boil the homogenized material for extraction anyway, this might be negligible, but check for glucosinolate decomposition first and keep all parameters constant. Maybe you could carry a small piece of material with known glucosinolate content as a control during this prozess.
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