I have an unknown sample, where isophthalic or terephthalic acid could be there, how I can identify the exact acid ?

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Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Can you help me with a free /no cost publication journal in oncology Nursing?

Wish to publish an article but in a cost-free journal. Need the assistance of researchers on good indexed journals free of predatory. Thank you

23 September 2020 5,372 3 View

How do you differentiate your different populations in your FASTA file in R ?

I need to identify my populations from my FASTA file in R so that R can then run all my stats. However, I do not know how to tell R how to group my data into 4 populations! This is the warning...

09 September 2020 8,875 2 View

How to generat a ASCII format data in GIS?

Dear you all: Recently i met with such a problem: i tryed to generate a ascii format buffer in gis, but i failed because it's headers are different from the research data. Failed result are shown...

11 August 2020 5,040 3 View

Do 3 light sources with same intensities, but different wavelengths(blue,green, red) provide same amount of energy for photosynthesis?

As it is not directly my field, I would like to ask for help in terms of the correlation between light intensity, wavelength, frequency and amplitude. Let's say, I have a radiophotometer which...

29 April 2020 9,141 2 View

How does one identify and extract statoliths from cubozoans?

Specifically, I am looking at the species Alatina alata. I am having trouble both finding the statoliths and also extracting them for further analysis. I have been freezing specimen before I am...

24 April 2019 7,943 2 View

Which test to use in SPSS?

So i have a study with both males and females, they will both be shown a negative image and their brain reaction recorded and a positive image with their brain reaction recorded what analysis in...

30 March 2019 4,571 6 View

Using floating dead cells in western blot analysis after drug treatment?

Hey guys, I need your help to decide if I should include floating dead cells in my western blot analysis. I am looking to check expression of certain proteins after drug treatment in adherent...

28 January 2019 4,354 10 View

Trouble with protein ladder in western blot. what could be the reason?

I am using 4-20% biorad precast mini gel and protein ladder is also from biorad. I have attached the image of membrane. Running voltage- 100 V for 1 hr at room temperature and 70 V for 2.30 hr at...

18 December 2018 4,231 5 View

How to solve a steady state homogenous problem in Matlab using Matrix approach?My results does not mimick the sytem under observation.Any suggestion?

I am solving a numerical modelling stem of autothermal reforming using MATLAB. The problem is ,Pressure and Temperature results are not stable (unsteady state problem) and escalate too much I...

06 July 2018 1,012 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View