I have a dilution consisting of nanoparticles and bacteria, how can I isolate nanoparticles?

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Amit Jaiswal

Centrifuge your sample at 5000 rpm; this will remove your bacteria as pellet. The remaining supernatant will mostly be of your nanoparticle.

After removing bacteria, You can try alcoholic precipitation to concentrate your nanoparticle, but am not sure whether this will be applicable for your case..

Regards

Amit

Dipak K Majumdar

Filter your sample through sterile 0.22 micron cellulose acetate membrane filter to remove bacteria.This is a standard method for sterilization by filtration.The filtrate will contain sterile nanoparticles.