I did zymography and I can see clear MMP2 bands. I also see pro-MMP-2 bands? How can PRO-MMP-2 have gelatinase activity? And why can't I see MMP-9?

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No title

I am medical student. I want robbins pathologie fr deutsch pdf . how am I find this book ?

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Richard Bauer

the reason you detect active and pro forms on a gelatine gel is, because you partially denature the pro forms during the separation process in gel electrophoresis. This way the active site of the pro-form is exposed , followed by cation-exchange, and that allows also the pro-forms to interact with collagen.