Hi! I was going through the literature on Phospho-Molybdate Assay (Total Anti-oxidant Capacity) and I was puzzled by the formula for the percentage anti-oxidant capacity which is given below
%Antioxidant effect =(control absorbance - sample absorbance/ control absorbance)*100
To the best of my understanding, in this assay the anti-oxidant capacity increases as the absorbance increases. This should mean the control sample should have the lowest absorbance and hence the lowest anti-oxidant capacity. It would also mean that if I were to use the formula given above, the numerator would come out as negative. Can we use negative values? Is the formula correct? Or is my understanding of the basic principle of the assay flawed?
%Antioxidant effect =(((absorbance at time t) - (absorbance at time =0))/ (absorbance at time =0)))*100
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