I am using qPCR method published by O'Callaghan and Fenech to measure leukocyte Telomere length. How can I calculate the length?

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Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

FinFET-10nm Technology File?

Dear Authors and Researchers, I'm looking for predictive technology models (PTM) for FinFET including 10nm. Unfortunately, the following webpages do not work...

14 April 2024 4,390 1 View

Is there any technical way to test stability analysis of wave function in QUANTUM ESPRESSO?

I did a branch of the DFT calculation to find the stable cell parameter (a) . I didn't get the smooth curve for energy(E) v.s a. There is some point out of concave curve that I had to repeat...

21 March 2024 2,037 2 View

How to increase concentration and quality of RNA after extraction?

Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...

11 March 2024 9,171 8 View

Why a new peak has appeared in the spectrum after dye adsorption?

Why after the adsorption of methyl orange dye by my adsorbent, in addition to the color peak in 464 nm, another peak has appeared in 372nm n in theuv-vis spectrum. It should be noted that this...

10 March 2024 4,167 2 View

How to interpret my samples' results from D1000 ScreenTape Assay?

Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...

10 March 2024 8,764 0 View

Can anyone help with SHSY5Y cells differntiation?

Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...

19 February 2024 1,672 5 View

Why Cryo cells (freezed cells) do not attached to the flask?

Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...

19 February 2024 9,305 2 View

Can someone suggest protocol to produce knock-down cells by using shRNA lentivirus particles?

Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...

10 January 2024 6,425 0 View

Is there any one here to contribute to the comparative paper?

I've had two articles published. Both are nearly identical, and I'd like to write a comparison article about their outcomes. For this, I'll need to use Comsol for simulation or machine...

27 November 2023 3,346 1 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

How to solve g_mmpbsa error?

Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out

07 August 2024 6,066 0 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

JCPDS 65-7246 file please?

Dear Researchers Kindly share JCPDS 65-7246 file Thanks in advance

04 August 2024 5,613 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

Difficulty with permittivitt and Magnetic Permeability Calculations?

Difficulty with permittivitt and Magnetic Permeability Calculations Hello everyone, I have all the parameters related to the calculations of the permittivitty and magnetic permeability...

30 July 2024 5,206 1 View

How to do pca analysis of c-alpha atom of the protein?

i m interested in pca analysis of c-alpha atoms in gromacs for that i used the following gmx_mpi covar -s mdca.tpr -f mdca.xtc -o eigenvalca.xvg -v eigenvecca.trr -av average.pdb -n index.ndx but...

30 July 2024 1,607 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View