Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Loss of biodiversity its impact on terrestrial ecosystem?

I need research papers with problem statement as well as research questions in respect of the above mentioned topics

04 February 2021 2,240 15 View

Where can I find data sets containing the average mass of terrestrial predators and their prey?

For my final year thesis I require data on the average body mass of terrestrial predators and their prey. I have come across many papers which have used such data sets however, the data is not...

04 February 2021 8,348 3 View

What does it mean to be a digital bystander in the world of learning, teaching and education?

Consider this vignette: during the COVID-19 pandemic a family are living in a big city where access to stable digital streams and affordable data bundles is not a problem. Confined to long periods...

22 October 2020 6,220 3 View

Do you think we have found the Holy Grail of astrobiology? Are we not alone anymore?

Do you think we have found the Holy Grail of astrobiology and finally come close to proving that we are not alone in the universe? "Jane Greaves from Cardiff University in the U.K. and several...

13 September 2020 3,835 17 View

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18 August 2020 3,955 11 View

Is the antenna designed for Ku band operating at 14.25GHz suitable for terrestrial communication? or suitable for RADAR communication?

I have designed an antenna to operate at 14.25 GHz( 14 to 14.5 GHz)uplink frequency of Ku band with linear polarization. what kind of application is suited for my designed antenna? and reference...

11 July 2020 699 2 View

What is the best way to register crop point clouds and spectral information in a plot of the field ?

Hi everyone, The goal: i want to register crop point clouds and spectral information in a plot of the field. The data detail: 1.The crop point clouds will be obtained by terrestrial Lidar (or UAV...

05 July 2020 9,128 5 View

Behaviour, inducing stress in terrestrial invertebrates?

Hi, I found a number of behavioural papers studying anxiety behaviour following stress. Stress was induced by exposure to predators, agonistic interaction or electric shocks. But these are almost...

24 June 2020 309 1 View

Developments in South african Family law?

16 June 2020 3,683 3 View

Corona impacts on the life and behavior of the people. Does, it will change the world, socially and economically?

Time witness several foul, hatred and inhuman cases between the different creeds and communities and also recorded many domestic violence inside the family during this pandemic lockdown.

14 May 2020 4,425 16 View