I have found a nice way (at least I think so) how to estimate collagen orientation in 180° range istead of usual 90° range without the necessity of using quarter wave plates (see attached figure-it shows orientation distribution of achilles tendon specimens with respect to lower horizontal edge of the image) but I am not an expert in the polarized light microscopy (PLM) so I can explain how it works but not why it works like that. Therefore I am looking for a help with the theory of PLM. I am preparing a paper for journals like Journal of Miscroscopy or Microscopy and Microanalysis and I am offering an authorship (2nd or 3rd position based on the portion of work). Is anybody interesting in cooperation with me? Thanks for your answers.
We have done a bit of work a few years ago in terms of polarised light microscopy and collagen - we never got round to publish it but i wonder if this is what you are after. contact me offline and we can discuss this in more details.
Dear Stan, I had a short discussion with a research colleague from the University of Reutlingen, but we did not completely understand your problem. The whole problem can be finally boiled down to matrix manipulations with so-called Müller matrices, where of cause symmetry conditions in e.g. apolar systems have to be taken into account. If we should continue, please contact me directly. All the best Bernd
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