What's the difference between 0.1% triton-X100 and ice-cold methanol in the immunofluorescence assay for intracellular protein staining?

Hi, Guys, I noticed that researchers usually permeabilized the cells with 0.1% Triton-X100 or ice-cold methanol (90%) for an intracellular protein staining, it looks like that the two methods work...

15 February 2021 5,606 4 View

How to wash the LiF left by Ti3AlC2 etching?

I added 1 g Ti3AlC2 to 1g LiF and 20mL 9 M HCl and stirred it at 450 rpm at room temperature for 48 h.After deionized water washing and ultrasonic treatment, the product was obtained, but its XRD...

08 October 2020 7,612 4 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View

I want code Matlab for color image denoising?

I want code Matlab for color image denoising

05 July 2020 150 4 View

No title

Please I need to know the pressure ?, relative proportions of Titanium and Nitrogen Imput in the plasma chamber?, Discharge curent density directed to samples? , sample voltage?, target voltage...

24 March 2020 1,409 3 View

Where can I submit a letter to the editor or correspondence article?

I am a medical school student in China, and I have written several short articles like 400-600 words regarding Chinese medical policy, medical education and so on. I focus on the violence against...

17 March 2020 5,527 3 View

Dissertation title for Msc in Cybersecurity and management?

I am looking for idea of doing dissertation in cybersecurity and management for Msc. Can someone help me to give the advice on dissertation title and sector and field to research ? Thank you.

25 November 2019 7,657 7 View

How can I overcome negative frequencies that occur during Gaussian calculations?

22 May 2017 3,421 5 View

Nanohardness of hard carbon films, Can any body measure the nanohardnesof my carbon based films?

Thank you

11 December 2015 5,080 4 View

How to fit the Nyquist impedance plot for obtaining the bulk resistance of a polymer film?

In general, the intersection of the high frequency side semicircle to the real axis gives the bulk resistance. This is quite easy, if the center of the semicircle lies on the real axis. But in...

22 January 2014 5,745 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View