How to clone pcDNA vector in SH-SY5Y cell line?

Dear all, How I can clone two genes in pcDNA vector, simultaneously (one of them is target gene with 500 bp length and one of them is GFP). Also, how we can transfect the vector into SH-SY5Y cell...

12 November 2020 6,122 3 View

How to simulate MSE walls for dynamic FE analyses?

Hi colleagues, Because of the complexity of modelling geogrid layers and their interfaces which can cause convergence problem in FE analyses, I have seen in practice they model the whole MSE wall...

14 August 2020 4,649 3 View

How I can see contact stress in my analysis result in abaqus ?

I want to report contact stress in my result, is that cpress (contact pressure) ? what is the different between contact stress and contact pressure? !

27 July 2020 3,767 3 View

Is it possible to simulate tip-sample interaction during Scanning Probe Microscopy in Abaqus? and if not which software do you recommend?

I want to simulate this interaction in Abaqus but I heard it may be possible only in Comsol, considering the samples are measured in Nano scale, thanks

01 April 2020 8,313 3 View

Does industrial scale Hydrometallurgical purification of Silicon exist?

There are several papers describing different hydro-metallurgical purification routes of Metallurgical Si to Solar Si through alloying, doping, nano sized grinding,... and finally leaching in...

28 March 2020 2,201 3 View

Why TiO2 powder earnd from sulfate process turns yellow after anealing?

Tio2 powder is earned after sulfate process by solving illmanite in the sulfuric acid and a purity of up 95% of TiO2 is gained . why the color of Tio2 turns yellow after 2h annealing in 900 oC...

07 February 2020 5,769 3 View

How to fine grind material in a narrow band particle size distribution?

Does anybody have ideas to mill Silica powder to micron size particles to a narrow particle size distribution (PSD)? For example grinding with planetary ball mill makes a wide PSD which is not...

19 December 2019 5,431 2 View

Hi all, could you please recommend a good review paper on the scheduling of renewable energy resources (RESs) in microgrids?

I would be very thankful if anyone could recommend some good review papers about the scheduling or economic dispatch of RESs in the microgrids. The assessment of the greenhouse gasses in the...

05 December 2019 6,335 3 View

Diffused dye front on SDS-PAGE, any suggestions on what the problem might be?

I ran these 15% gels for 90 minutes under 200 volts ( gel ran too slow), ladder looked very condense on the top and the dye front was somehow diffused at the bottom of the gel. I am sure about the...

16 April 2019 6,092 5 View

Local outlier detection mechanism implementation in matlab ?

i want code for implementation of local outlier detection mechanism in matlab for addressing slow http problem.

21 October 2018 5,023 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View