Why doesn't the simulation diverge, while the auto-shutoff level (in Lumericl FDTD) increases?

Hi, I try to simulate a graphene-based structure. At the beginning of the simulation, the auto-shutoff level decreases, but, approaching the end, the auto-shutoff level increases, while the...

16 April 2020 4,807 3 View

Are Turbiscan Lab data exportable into an excel file?

I'm using the Turbiscan Lab Instrument to assess shelf life and stability properties of my dispersions at different temperatures. However, once analysing my data, I can only export the graphs...

23 March 2020 8,028 3 View

What is expression mean of Terminated by Solver in GAMS?

I use CPLEX for solving a MIP problem and I stop my run. I receive message of Terminated by Solver in Solver Status.

02 December 2019 4,067 1 View

Why I receive error when evaluating envelope correlation coefficient By CST?

hello everyone, I designed two-port antenna by CST 2018. and it was simulated correctly. but when evaluating envelope correlation coefficient I receive error like below: Template based...

28 June 2019 3,635 3 View

EEG or FMRI data?

Hi everybody. I plan to use complex network analysis methods to investigate psychiatric disorders. Most researchers have used FMRI data to construct brain network. I wonder if it is possible to...

16 December 2018 7,634 4 View

Dose anyone know how I can treat cell clumps in neural stem cell culture to get neurospheres?

Hi all, I am working with neural stem cells from SVZ in mice. Usually I get these cells from 4-7 days neonatal mice. this time I got cells from 11 days neonatal and result of my cell culture is...

30 July 2017 2,351 3 View

White blood cells culture and RNA extraction from them

22 July 2017 2,501 2 View

Can we use FACS Calibure flow cytometer in order to detect CD 51antibody with material number bs-2250R-A350?

07 August 2016 1,772 3 View

How can I justify a drop in viscosity of alginate solutions by addition of monovalent salts such as sodium chloride?

21 January 2016 4,337 3 View

Are there any practical strategies to get less confident students to talk? both for elementary and intermediate students?

I have classes with both EFL intermediate and lower intermediate students; how can I encourage them to talk esp. those who are less confident

22 August 2015 1,093 45 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

Ac16 cardiomyocytes cell line culturing ?

I have faced a problem with culturing Ac16 cardiomyocytes cell line. Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM. Day 3 I put my cells on Low serum medium. When I changed...

24 January 2021 1,456 2 View

Is there any method I can use to isolate single cells (i.e cardiomyocytes and non-cardiomyocytes) from snap from rat heart tissue?

I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...

19 January 2021 4,044 3 View

How to isolate human cardiac stem cells? Which other cell types could be in this cell culture?

Hello, I followed a protocol for isolating human cardiac stem cells (Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human...

18 November 2020 2,928 3 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

Are there reliable antibodies against TRP channels in cardiac cells ?

From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or...

07 May 2020 6,865 1 View

Monitoring ATP binding to proteins: TNP-ATP or MANT-ATP?

Hi all, I'm interested on monitoring ATP binding to a protein of interest using fluorescent ATP analogs, such as TNP or MANT-ATP. Do you have any experience with them? Which one is the best...

03 May 2020 3,993 5 View

ATP content measurement?

Hello, I am looking for a clear protocol for ATP content assay. I am not looking for a kit because it is expensive. I am looking for a fluorogenic or a colorimetric substrate that helps me to...

24 March 2020 2,286 3 View