I have to use hydrogen peroxide to verify the redox state of an organic cofactor by LC-MS measurement. However I have realized that commercial H2O2 contains stabilizers to reduce decomposition to water and oxygen under normal storage conditions. Common stabilizers, according to this website (http://www.h2o2.com/faqs/FaqDetail.aspx?fId=11) are sodium pyrophosphate, Organophosphonates, nitrate, and phosphoric acid and silicates.
At least the acids would be ok, but would someone have an idea about their behaviour on a C18 column coupled to an Orbitrap? Just checking if this is a good idea for direct injection; since I'm working with limited ammounts of material avoiding pre clean-up by SPE would be desirable.
Thanks a lot for your input!
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085011/pdf/nihms267353.pdf
https://patentimages.storage.googleapis.com/61/10/f8/8be530bc47f95f/WO2002026277A2.pdf
https://www.cir-safety.org/sites/default/files/hydper122018FR.pdf
Thanks
Mr. Pena,
Without preliminary sample pretreatment the quantitative method is described in the following reference and corresponding subreference sections. The quantification is carried out per span of the scan time. Equation (2) expresses the quantitative relation between the intensity of an MS peak and the concentration in solution. The method has significant capability comparing with the known approaches, because of the total intensity does not correlate exactly to the concentration of the analyte in solution. The LOD is at about fmol range.
Conference Paper Mass spectrometric diffusion parameters and 3D structural an...
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