We have a HPLC preparative shimadzu. We have the LC connected to a PDA module. Everything looks fine, so LC is ready and PDA is also ready. Once we inject, we see that the PDA is changing to Standby modus and from there it does not change once the LC is running. So, that means that we can not get PDA information.
Does anyone knows this problem?
PDA gives you an advantage of checking your data at variable wavelengths in the offline mode that means after running your sample you can get chromatogram at different wavelengths within the range you have fixed in PDA. So first fix the range & then run the sample and check the data.
Dear Rogerio,
do you use the LCSolution software? One classical mistake can be the adjustment of the run time. When writing the method, also the window of the PDA requires to fill in the LC run time. Second cause of the problem could be the start signal. The PDA requires an external start signal from the autosampler or CBM. Make sure that a two core cable installed properly and check if the relay in the configuration window is set to "start".
Good luck.
Thank's for the help!
We found the problem. Was a wrong software configuration . On the System controller we need change the configuration on the relay, that was Event on 1, 2, 3 and 4; and is necessary to be start. As Benedickt said.
Thank you again!
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