One of our synthesized nanoparticles have the fluorescence quenching ability. While we have conjugated with any fluorescent dye or fluorescent drug, the fluorescence activity is going to be quenched. Is it possible to use this property for sensors or any other applications? Please suggest me.
If quenching is reversible, can withstand prolonged cycling...Yes, optical and SERS sensors are possible with your NPs.
Actually, this phenomenon is typically used to detect gaseous oxygen, among other applications. The magnitude to measure has to quench the emission, as Sridhar Vadahanambi points, in a reversible way.
For certain sensing applications, Stern-Volmer equation models this behavior, showing a linear relation-ship between the emission intensity and the concentration of the analyte to detect.
Yes you can use it in sensor applications. As Elosua Cesar mentions in oxigen sensing, but also in pharmaceutical compounds or amines.
You may try for VOC detection.
Could you suggest any fluorescence material to detect VOCs I'm very interested in that field!
Nanoparticles easily bind with drugs, dyes, organic molecules. so you may take different concentrations of nanoparticles ( 0.001 to 0.00001) with 0.0001 (or) 0.00001 concentration of the dyes, etc. then you should measure the absorption maximum of the solution. the absorption maximum should be fixed as the excitation wavelength. then you measure the fluorescence intensity of the emission wavelength. the flu. intensity may deceased at the same wavelength. this is fluorescence quenching.
Answer to Elosua Cesar:
Yes, hybrids based on polymers-porphyrin, or on silver colloids-porphyrins.
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